T5941

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T5941

Sigma

 

Trizma® hydrochloride

for molecular biology, Biotechnology Performance Certified, cell culture tested, ≥99.0% (titration)

Synonym:TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride
CAS Number:1185-53-1
Linear Formula:NH2C(CH2OH)3 · HCl
Molecular Weight:157.60
Beilstein Registry Number:3675235
EC Number:214-684-5
MDL number:MFCD00012590
PubChem Substance ID:24900332

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Description

ApplicationThe pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Other NotesEasily compare specifications for Trizma HCl products with the Trizma HCl specification table.
Legal InformationTrizma is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Properties

gradeBiotechnology Performance Certified
 for molecular biology
assay≥99.0% (titration)
total impurities DNase, RNase, protease, none detected
  Endotoxin and bioburden, tested
 ≤0.5% water (Karl Fischer)
useful pH range7.0 - 9.0
pKa (25 °C)8.1
EQP levelElite
mp150-152 °C
solubilityH2O: 667 mg/mL
cation tracesheavy metals (as Pb): ≤5 ppm
abs.A40%/290 ≤0.05
suitabilitycell culture tested

Safety

Personal Protective Equipmentdust mask type N95 (US), Eyeshields, Gloves
Hazard CodesXi
Risk Statements36/37/38
Safety Statements26-36
WGK Germany1

References

referenceCull, M. and McHenry, C.S., Preparation of extracts from prokaryotes. Meth. Enzymol. 182, 147-153, (1990) Abstract
BeilsteinBeil. 4,H,304